Research Technician

The faculty in MBM are united by a common interest in the biology of microbes (bacteria, fungi, viruses, and parasites) and the effects of microbes on human and animal hosts. The microbial pathogens program studies microorganisms that cause infectious diseases, with emphasis on rigorous analysis of these pathogens and the immune responses that restrict them. Program investigators direct detailed studies of pathogenic bacteria, viruses, fungi and eukaryotic parasites. Research topics include structural analysis of virus entry, viral evolution, viral oncogenes, intracellular bacterial pathogens, microorganisms that cause diarrhea and pneumonia, intracellular signaling, pathogen adhesion, genetic determinants involved in susceptibility to infection, vaccine development, and identification of novel antimicrobial agents.

The major functions of this position are to conduct experiments and to organize the laboratory. Duties will include: use of murine models of oropharyngeal candidiasis and of voluntary ethanol consumption to study effects of fungi on oral disease and murine drinking behavior, isolation of C. albicans strains from human specimens, analysis of fungal and host gene expression, ordering supplies, maintaining required paperwork to ensure regulatory compliance. Past technicians have been co-authors on publications from the laboratory. The successful candidate must be personable and extremely well organized and have a BA or BS in a scientific discipline. Training will be provided for the techniques required to conduct the research project.

Essential Functions:

• Perform studies of C. albicans in mice:
  
  • handle mice
  • inoculate mice with microorganisms
  • evaluate outcome of inoculation
  • keep accurate records of experiments and result
• Culture C. albicans strains from human specimens:
  
  • plate on selective media
  • characterize phenotypes
• Analyze gene expression
  
  • RNA extraction from tissue or laboratory culture
  • measure expression by RT qPCR
• Manage the laboratory including (but not limited to):
  
  • order supplies and maintain inventory
  • maintain laboratory equipment for optimal productivity
  • perform assigned lab maintenance duties on a regular basis
• This description is not intended to be all-inclusive. Employee may perform other duties as assigned to meet the ongoing needs of the organization.

Basic Requirements:

• Knowledge and skills as typically acquired through Bachelor's degree in a Biological science or Chemistry, with up to one year of laboratory experience.
• Proficient in the use of Microsoft Office and PRISM software.
• Must be able to fluently communicate, read, and write in the English language.
• Self-motivation, consistent attention to detail, and excellent organizational, communication, and time management skills are very important.

Preferred Qualifications:

• Previous experience with animal modeling, working with microbiology and/or molecular biology are preferred but not required.